Journal: Blood cancer discovery
Article Title: An Autochthonous Mouse Model of Myd88- and BCL2-Driven Diffuse Large B-cell Lymphoma Reveals Actionable Molecular Vulnerabilities
doi: 10.1158/2643-3230.BCD-19-0059
Figure Lengend Snippet: A) Exemplary images of PLA assays for MYD88 proximity with IRAK4, IRAK1, BTK and IgM, respectively. Blue, DAPI; red, PLA signal. Scale bars represent 10 μm. B). Quantification of the data shown in A). Each dot represents the average number of PLA signals per cell and experimental well. The mean and SEM of at least three independent experiments are depicted. Significant differences between samples was calculated by Welch’s unpaired t-test. C) Lysates were generated from CD43-depleted splenocytes from 10 weeks old MC and WT. Immunoblots were generated for the indicated targets. Blots were quantified using ImageJ.
Article Snippet: B cells were isolated from spleens of 15 - 20-week old wt and MC mice by MACS sorting according to protocol (CD43 (Ly-48) Micro beads, Miltenyi Biotec, Cat. No. 130-049-801).
Techniques: Generated, Western Blot